منابع مشابه
A nuclease from mung bean sprouts.
For some years now a systematic survey of deoxyribonucleases has been carried out in this laboratory in the hope that a deoxyribonuclease would be found with limited, yet well defined, specificity. A preliminary note of Masui, Hara, and Hiiamatsu (2) described an apparently labile DNase from soy bean sprouts which liberated first deoxyadenylic, then deoxyguanylic, acids. We encountered difficul...
متن کاملMung Bean Nuclease I III . PURIFICATION PROCEDURE AND ( 3 ’ ) ~ o - MONOPHOSPHATASE ACTIVITY
A method leading to partial purification of mung bean nuclease I (potency 250) in good yield is described. Mung bean nuclease I of potency 1800 was obtained in small yield. At this stage the preparation showed two bands on disc electrophoresis. Throughout all stages of purification the nuclease activity was accompanied by (3’)~w-monophosphatase activity, suggesting that both activities are intr...
متن کاملMung Bean Nuclease Treatment Increases Capture Specificity of Microdroplet-PCR Based Targeted DNA Enrichment
Targeted DNA enrichment coupled with next generation sequencing has been increasingly used for interrogation of select sub-genomic regions at high depth of coverage in a cost effective manner. Specificity measured by on-target efficiency is a key performance metric for target enrichment. Non-specific capture leads to off-target reads, resulting in waste of sequencing throughput on irrelevant re...
متن کاملMung Bean (Vigna radiata) Seedlings'
Two forms of Fd-NADP+ oxidoreductase (FNR) isoproteins have been purified and characterized from the first foliage leaves of 5d-old mung bean (Vigna radiata). They could be separated by either Mono Q HR 515 or ferredoxin (Fd)-Sepharose 48 affinity columns. Based on immunoblot analysis and N-terminal amino acid sequences, one form resembles the FNR purified from photosynthetic tissues of higher ...
متن کاملInduction of double-strand breaks by S1 nuclease, mung bean nuclease and nuclease P1 in DNA containing abasic sites and nicks.
Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of the enzymes acted on the substrate containing discrete abasic sites. Under conditions where little or no non-specific DNA degradation was observed, all three nuc...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63349-6